227-32805-04_A_edited
227-32805-04_A_edited

Shim-pack Arata C18, 5um, 150mm x 4.6mm

Part Number: 227-32805-04

Particle size (µm): 5

ID (mm): 4.6

Length (mm): 150

USP: L1

Functional Group: C18

Mode: Reversed Phase

Type: Analytical column

Pore size (nm): 12

Surface Area (m2/g): 340

End Capping: Yes

Carbon Load (%): 17

PH: 2 - 7.5

Pressure Tolerance (MPa): 20

Pack size: 1

Column Name: Arata C18

Unprecedented Resolution and Peak Shape of Basic Compounds

Even for LC columns that claim to be designed for basic compounds, adequate resolution often can not be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase.

All of these issues have been solved with Shim-pack Arata that was specifically designed to give unmatched peak shape for basic compounds.

Unmatched Peak Shape Elegantly Designed for A Wide Range of Compounds

Unmatched peak shape of basic compounds could be achieved while maintaining good peak shape for acidic compounds with Shim-pack Arata LC columns. Even with low ionic strength acidic mobile phase, such as 0.1% formic acid containing mobile phase, excellent peak shape of both amitriptyline (a basic compound) and benzoic acid (an acidic compound) could be achieved.

Excellent Separation Performance for Peptides Even with Weak Ion Paring Acids

In order to obtain good peak shape of peptides under reversed phase chromatography, TFA containing mobile phases are frequently used which the ion pairing effect is relatively strong. However, TFA could cause ion suppression in LC/MS (/MS) analysis. Excellent peak shape and separation performance for peptides could be achieved on the Shim-pack Arata LC column even with 0.1 % formic acid (weak ion paring acid) containing mobile phase.

 

Rapid Equilibration (Even with Low Ionic Strength Acidic Mobile Phases)

When analyzing basic compounds on a typical ODS column with low ionic strength acidic mobile phase, peak shape and long equilibration times are common problems. Shim-pack Arata LC columns can be rapidly equilibrated in low ionic strength acidic mobile phases yielding excellent peak shape and stable retention times.

 

*Both were new columns (shipping solvent : acetonitrile) and equilibrated with mobile phase without any conditioning.
Basic drugs were analyzed after a certain period of time of equilibration and RT and symmetry factor of the drugs were compared.

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