STREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS
EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products.
- Glycan Release Reagent A
- Glycan Release Reagent B
- Glycan Capturing Beads
- Filter Column
- Reducing Reagent
- Cleanup Column
O-GLYCAN SAMPLE PREP IN 5 HOURS
O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day.
A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins.
Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy.
EASY AND SAFE OPERATION
With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.
Part No.: APASBC-BSX4410Z
HPLC-Ready N-glycan Preparation The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant. The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant. Contents Antibody Capturing Column (Qty 10) Silicone Bottom Cap (Qty 10) Antibody Capturing Solution (Qty 1) Washing Buffer (Qty 1) Glycan Release Enhancer (Qty 1) PNGase F Solution (Qty 1) 2-Aminobenzamide (Qty 3) Reducing Reagent (Qty 3) Cleanup Column (Qty 10) Instruction Manual (Qty 1)